|
| Status |
Public on Apr 04, 2010 |
| Title |
E10.5 wild-type cortex, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 E10.5 cortices
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embryonic brain cortex
genotype: wild type
genetic background: C57BL/6
age: E10.5
|
| Treatment protocol |
Lysates were gently homogenized in cold PBS and then Trizol and frozen at -80C
|
| Growth protocol |
E10.5 embyros were harvested from pregnant females and cortices (pair per embyro) were carefully dissected and placed on ice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed accordeing to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Per RNA labeling, 300 nanograms of total RNA was used in conjunction with the Affymetrix recommended protocol for the GeneChip 1.0 ST chip
|
| |
|
| Hybridization protocol |
Labelled and fragmented cDNAs were hybridized to Affymetrix Mouse GeneChip 1.0 ST chips overnight. The chips were washed and stained by the Affymetrix Fluidics Station.
|
| Scan protocol |
Genechips were scanned using an Affymetrix Gene Chip Scanner 3000.
|
| Description |
n/a
|
| Data processing |
Gene expression intensities were caluculated using Gene expression intensities were calculated using GeneChip® Command Console® Software (AGCC) and Expression Console™ Software. RMA normalziation was performed to generate the CHP files. Genesifter software and Pairwise comparisons were perfomed with Benjamini and Hochberg correction.
|
| |
|
| Submission date |
Nov 24, 2009 |
| Last update date |
Mar 30, 2010 |
| Contact name |
Christopher Pan |
| E-mail(s) |
cpan@mail.nih.gov
|
| Organization name |
NIH
|
| Department |
NHGRI
|
| Street address |
Rm 5228, Bldg 50, South Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE19168 |
Expression profiling analysis of mouse E10.5 Magoh mutant brain cortices |
|
