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Sample GSM475316 Query DataSets for GSM475316
Status Public on Nov 21, 2010
Title control liver 3
Sample type RNA
 
Source name virgin dam
Organism Rattus norvegicus
Characteristics treatment: control
tissue: liver
Treatment protocol Day 10-11 lactating Sprague-Dawley rats or virgin controls were sacrificed and whole liver, duodenum, jejunum, and ileum homogenates were used for RNA extraction.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol reagent followed by purification with Quiagen's RNAeasy kit.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) according to the manufacutrer's instructions.(www.Affymetrix.com)
 
Hybridization protocol Samples were hybridized with GeneChip Rat Exon 1.0 ST Arrays (Affymetrix) and scanned at the Uiversity of Kentucky Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Expression Console software; extended-gene analysis
Data processing .CEL files were analyzed in Affymetrix Expression Console(EC) using the RMA algorithm on the Extended dataset to background correct, normalize, and log 2 transform the dataset.
 
Submission date Nov 24, 2009
Last update date Oct 11, 2010
Contact name Mary Vore
E-mail(s) maryv@email.uky.edu
Phone 8593235249
Organization name University of Kentucky
Department Graduate Center of Toxicology
Street address 1095 Va Drive
City Lexington
State/province KY
ZIP/Postal code 40536
Country USA
 
Platform ID GPL6543
Series (1)
GSE19175 Differential gene expression in lactating rats compared against age matched virgin controls

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
7045119 6.44859
7094268 3.21021
7110652 5.69006
7176187 6.19089
7225339 3.94345
7061498 6.28297
7143418 2.46625
7258105 12.2178
7225336 3.71814
7127031 3.60094
7225334 2.60247
7110645 3.97729
7143412 5.26801
7258100 5.75807
7323636 2.36186
7159795 1.789
7225331 2.11397
7028722 1.97827
7274482 4.86537
7323634 6.20882

Total number of rows: 19434

Table truncated, full table size 301 Kbytes.




Supplementary file Size Download File type/resource
GSM475316.CEL.gz 21.7 Mb (ftp)(http) CEL
GSM475316.chp.gz 141.2 Kb (ftp)(http) CHP
Processed data included within Sample table

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