|
| Status |
Public on Nov 21, 2010 |
| Title |
control ileum 2 |
| Sample type |
RNA |
| |
|
| Source name |
virgin dam
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: control
tissue: ileum
|
| Treatment protocol |
Day 10-11 lactating Sprague-Dawley rats or virgin controls were sacrificed and whole liver, duodenum, jejunum, and ileum homogenates were used for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent followed by purification with Quiagen's RNAeasy kit.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) according to the manufacutrer's instructions.(www.Affymetrix.com)
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Rat Exon 1.0 ST Arrays (Affymetrix) and scanned at the Uiversity of Kentucky Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; extended-gene analysis
|
| Data processing |
.CEL files were analyzed in Affymetrix Expression Console(EC) using the RMA algorithm on the Extended dataset to background correct, normalize, and log 2 transform the dataset.
|
| |
|
| Submission date |
Nov 24, 2009 |
| Last update date |
Oct 11, 2010 |
| Contact name |
Mary Vore |
| E-mail(s) |
maryv@email.uky.edu
|
| Phone |
8593235249
|
| Organization name |
University of Kentucky
|
| Department |
Graduate Center of Toxicology
|
| Street address |
1095 Va Drive
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536 |
| Country |
USA |
| |
|
| Platform ID |
GPL6543 |
| Series (1) |
| GSE19175 |
Differential gene expression in lactating rats compared against age matched virgin controls |
|
