|
| Status |
Public on Jan 05, 2012 |
| Title |
Uncontrolled asthma UA3 |
| Sample type |
RNA |
| |
|
| Source name |
Nasal airway epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Uncontrolled asthma
tissue: Nasal airway epithelium
gender: Male
age: 9.81 y
|
| Treatment protocol |
Nasal airway epithelial cells were brushed from children and immediately lysed with 100 µl of Absolutely RNA® Microprep Kit lysis buffer mixed with 0,7 µl of ?-Mercaptoethanol 14,2M then stored at -70°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Absolutely RNA® Microprep Kit extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 3ug of DNA were hybridized for 17 hr at 45C on Huge gene 1.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from dust mite allergic child nasal brushing with rhinitis + asthma
|
| Data processing |
The data were analyzed with Expression Console using Affymetrix default analysis settings and Gene Level-RMA sketch as normalization method.
|
| |
|
| Submission date |
Nov 25, 2009 |
| Last update date |
Jan 05, 2012 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE19187 |
Nasal epithelium gene expression profiling in child respiratory allergic disease |
| GSE19190 |
Distinct epithelial gene expression phenotypes in childhood respiratory allergy |
|
