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Sample GSM476670 Query DataSets for GSM476670
Status Public on Dec 02, 2009
Title preB.FrD.BM#3
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics age: 8 weeks
exclusion markers: CD3 Ly6c Ter119 Gr1 Mac1
genetic background: C57BL/6J
phenotype markers: CD19+ IgM- CD45R+ CD43-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol disruption by 1ml syringe, ACK lysis of RBCs
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description preB.FrD.BM#3, Fr D (pre-B)
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 01, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.9452
10344622 304.9448
10344624 383.2538
10344633 823.0484
10344637 196.2952
10344653 19.7979
10344658 223.4237
10344674 12.2809
10344679 50.2686
10344707 336.8777
10344713 406.1264
10344723 111.0377
10344725 48.8731
10344741 3322.1
10344743 35.1772
10344750 192.6142
10344772 28.1945
10344789 251.3279
10344797 445.9683
10344799 321.3637

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM476670_EA07068_56612_MoGene_preB.FrD.BM_#3.cel.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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