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Sample GSM476686 Query DataSets for GSM476686
Status Public on Dec 02, 2009
Title Tgd.vg2-.Sp#3
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 5 weeks
exclusion markers: CD4- CD8- NK1.1-
genetic background: C57BL/6J
phenotype markers: TCRd+ Vg2- CD44-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Tissue Grinder MACS depletion / Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Tgd.vg2-.Sp#3, SpleenVg2-, resting
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 01, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 25.7304
10344622 306.852
10344624 332.2528
10344633 696.7172
10344637 195.3309
10344653 11.6879
10344658 319.4245
10344674 15.1182
10344679 49.5953
10344707 379.5426
10344713 321.7856
10344723 189.7143
10344725 56.7301
10344741 2565.8821
10344743 27.3958
10344750 337.4975
10344772 22.6438
10344789 214.3936
10344797 332.7681
10344799 191.9927

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM476686_EA07068_54552_MoGene_Tgd.vg2-.Sp_#3.cel.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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