|
| Status |
Public on Mar 29, 2010 |
| Title |
Induced sputum, normal FEV1/FVC, Asthmatic child 07 |
| Sample type |
RNA |
| |
|
| Source name |
Induced sputum cells obtained during an exacerbation.
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: Asthmatic child with normal FEV1/FVC
|
| Treatment protocol |
Induced sputum cells were processed in 0.1 % Dithiothreitol on a shaking water bath at 37 °C for 15 minutes with intermittent aspiration via pipetting every 5 minutes.
|
| Growth protocol |
Asthmatic children were instructed to visit the study center within 24 h of experiencing an exacerbation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from total sputum cells using TRIzol followed by RNeasy.
|
| Label |
biotin
|
| Label protocol |
The samples were labelled according to standardized protocols and reagents from Affymetrix.
|
| |
|
| Hybridization protocol |
The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols and reagents from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
|
| Description |
none
|
| Data processing |
The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
|
| |
|
| Submission date |
Jan 14, 2010 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Anthony Bosco |
| E-mail(s) |
Anthony.Bosco@telethonkids.org.au
|
| Organization name |
Telethon Kids Institute
|
| Street address |
100 Roberts Road
|
| City |
Subiaco |
| State/province |
WA |
| ZIP/Postal code |
6008 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE19903 |
Expression in induced sputum during acute exacerbations in asthmatic children with/without chronic airflow obstruction |
|
