|
| Status |
Public on Dec 15, 2021 |
| Title |
tumor-hydroxychloroquine-3 |
| Sample type |
RNA |
| |
|
| Source name |
tumor from hydroxychloroquine treated group
|
| Organism |
Mus musculus |
| Characteristics |
treatment: HCQ
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from tumor tissue by homogenization in trisol reagent, followed by purification with E.Z.N.A. RNA isolation (omega biotek)
|
| Label |
biotin
|
| Label protocol |
Total RNA (250 ng) was used to synthesize fragmented and labeled sense-strand cDNA and hybridized onto the Affymetrix Clariom S peg plate. The Affymetrix GeneChip® WT PLUS Reagent Kit Manual was followed to prepare the samples. the GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate sense-strand cDNA from total RNA. Following synthesis of sense-strand cDNA, the cDNA was fragmented and labeled with the kit. Fragmented and labeled cDNA was used to prepare a hybridization cocktail with the Affymetrix GeneTitan Hybridization Wash and Stain Kit for WT Arrays
|
| |
|
| Hybridization protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate was carried out using the Affymetrin GeneTitan MC Instrument.
|
| Scan protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate was carried out using the Affymetrin GeneTitan MC Instrument.
|
| Data processing |
Transcriptome Analysis Console Software v 4.0 was used for basic data analysis and quality control.
|
| |
|
| Submission date |
Dec 16, 2020 |
| Last update date |
Dec 15, 2021 |
| Contact name |
michael francis coleman |
| E-mail(s) |
mcoleman@unc.edu
|
| Phone |
9199850347
|
| Organization name |
UNC
|
| Street address |
235 dauer drive
|
| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (1) |
| GSE163348 |
Inhibition of IGF1R/IR and autophagy as an intervention in PDAC |
|
