|
| Status |
Public on Jan 22, 2010 |
| Title |
poxn homozygous mutant proboscis_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
proboscis RNA
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: male
genotype: homozygous
|
| Treatment protocol |
Proboscises were dissected and homogenized in Trizol in several batches for each biological replicate.
|
| Growth protocol |
male flies were reared for 8-18 days post eclosure at room temperature.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Twice amplified biotinylated cRNA was prepared from about 50ng total RNA.
|
| |
|
| Hybridization protocol |
cRNA was hybridized for 16h at 45oC to Drosophila 2.0 arrays. Affymetrix fluidics station 450 was used for washing and staining.
|
| Scan protocol |
Affymetrix scanner 3000 was used to read arrays.
|
| Description |
none
|
| Data processing |
data was processed and normalized using GC-RMA
|
| |
|
| Submission date |
Jan 21, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Peter Cameron |
| Organization name |
UC-Berkeley
|
| Street address |
GL71 Koshland, UC-Berkeley
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3200 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE19984 |
Gene expression analysis of Drosophila melanogaster taste tissue |
|
| Relations |
| Reanalyzed by |
GSE119084 |
