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| Status |
Public on Apr 13, 2021 |
| Title |
P12707_137 |
| Sample type |
SRA |
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| Source name |
Left heart ventricle
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| Organism |
Sus scrofa |
| Characteristics |
tissue: Left heart ventricle
sample location: lateral
swine id: 1640
timepoint: Day 14
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNAdvance Tissue kit with modifications: Reduce reagent volumes by dividing with 13. Use vortexing + centrifugation to homogenize samples.
SMARTer Pico
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
STAR for mapping reads to genome
featureCounts for counting genes
DESeq2 for count normalization and transformation
Genome_build: Sscrofa11.1
Supplementary_files_format_and_content: count matrix of reads mapped to genes
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| Submission date |
Jan 16, 2021 |
| Last update date |
Apr 13, 2021 |
| Contact name |
Staffan Holmin |
| Organization name |
Karolinska Institutet
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| Street address |
Visionsgatan 4, J5:20
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| City |
Solna |
| State/province |
Non US/Canada |
| ZIP/Postal code |
17164 |
| Country |
Sweden |
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| Platform ID |
GPL26351 |
| Series (1) |
| GSE164962 |
RNA-seq data from submiligram porcine myocardial tissue before and after myocardial infarction |
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| Relations |
| BioSample |
SAMN17359067 |
| SRA |
SRX9862478 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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