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Status |
Public on Apr 13, 2021 |
Title |
P12707_209 |
Sample type |
SRA |
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Source name |
Left heart ventricle
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Organism |
Sus scrofa |
Characteristics |
tissue: Left heart ventricle sample location: apical swine id: 1644 timepoint: 0 - 1 h
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Extracted molecule |
total RNA |
Extraction protocol |
RNAdvance Tissue kit with modifications: Reduce reagent volumes by dividing with 13. Use vortexing + centrifugation to homogenize samples. SMARTer Pico
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
STAR for mapping reads to genome featureCounts for counting genes DESeq2 for count normalization and transformation Genome_build: Sscrofa11.1 Supplementary_files_format_and_content: count matrix of reads mapped to genes
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Submission date |
Jan 16, 2021 |
Last update date |
Apr 13, 2021 |
Contact name |
Staffan Holmin |
Organization name |
Karolinska Institutet
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Street address |
Visionsgatan 4, J5:20
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City |
Solna |
State/province |
Non US/Canada |
ZIP/Postal code |
17164 |
Country |
Sweden |
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Platform ID |
GPL26351 |
Series (1) |
GSE164962 |
RNA-seq data from submiligram porcine myocardial tissue before and after myocardial infarction |
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Relations |
BioSample |
SAMN17359026 |
SRA |
SRX9862447 |
Supplementary data files not provided |
SRA Run Selector![Help](/coreweb/images/long_help4.gif) |
Raw data are available in SRA |
Processed data are available on Series record |
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