|
| Status |
Public on Jun 01, 2010 |
| Title |
LD_muscle_120d_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
longissimus dorsi, feotal, 120 day
|
| Organism |
Ovis aries |
| Characteristics |
developmental stage: 120 d
|
| Treatment protocol |
LD muscle was dissected from each animal at a pre-determined site within 15 minutes of euthanasia, snap frozen under liquid nitrogen and stored at -80oC.
|
| Growth protocol |
Animals were reared and euthanased in accordance with the animal ethics guidelines of Utah State University (Utah, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Genomer Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, GCOSvs1.4
|
| Description |
Gene expression data from Dorset/Suffolk/Rambouillet cross-bred sheep foetuses at 120 days development from muscle type (LD)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
|
| |
|
| Submission date |
Jan 29, 2010 |
| Last update date |
Jan 29, 2010 |
| Contact name |
Ross L Tellam |
| E-mail(s) |
Ross.Tellam@csiro.au
|
| Phone |
07 3214 2476
|
| Organization name |
CSIRO Animal, Food and Health Sciences
|
| Department |
Level 6 Queensland Biosciences Precinct
|
| Lab |
University of Qld.
|
| Street address |
306 Carmody Road St.Lucia
|
| City |
Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4067 |
| Country |
Australia |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE20112 |
Expression data from Sheep longissimus dorsi (LD) muscle during development |
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