|
| Status |
Public on Feb 03, 2021 |
| Title |
Saline control1 |
| Sample type |
RNA |
| |
|
| Source name |
Mice lung from PBS control
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
age: 6-8 week old
treatment: saline control lung
tissue: lung
|
| Treatment protocol |
For Th1-Th17 asthma model, mice were sensitized with 50 μg HDM (Greer Laboratories) and 0.5 μg c-di-GMP (Biolog Inc.) on days 1, 3, and 5 intranasal. After 5 days of rest, mice were subjected to 3 challenge sets each with 3 consecutive challenges with HDM & c-di-GMP with a rest of 3 days between each set. The c-di-GMP and HDM was instilled only on the first day of each challenge set, followed by HDM. Similarly, Th2 model followed the same sensitization and challenge protocol where 50 μg HDM was instilled except c-di-GMP.
|
| Growth protocol |
Six to eight weeks old naïve C57BL/6 J mice were purchased from Jackson Laboratory (Bar Harbor, MA) and all animal procedures and experimental protocols were approved by the Auburn University Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Invitrogen PureLink RNA Mini Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Array protocol (GeneChip Whole Transcriptome Array, Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
After fragmentation and terminal labelling, cRNA were hybridized on GeneChip Mouse Genome Array (Further GeneChip was washed and stained in Fluidics Station 450)
|
| Scan protocol |
GeneChips Array scanning was performed according to the manufacturer's instruction.
|
| Description |
Gene expression data from saline control lung
|
| Data processing |
Data was processed using Transcriptome Analysis Console (TAC) 4.0.1 instructions.
#%result_name=Auburn_1
#%array_type=Clariom_S_Mouse_HT
#%annotation=Clariom_S_Mouse_HT.r1.na36.mm10.a1.transcript.csv
#%comparison=1 vs 2
|
| |
|
| Submission date |
Feb 02, 2021 |
| Last update date |
Feb 03, 2021 |
| Contact name |
Amarjit Mishra |
| E-mail(s) |
amarjit.mishra@auburn.edu
|
| Phone |
334-844-2603
|
| Organization name |
Auburn University
|
| Department |
Pathobiology
|
| Lab |
Laboratory of Lung Inflammation
|
| Street address |
1130 Wire Road, 264 Greene Hall
|
| City |
Auburn |
| State/province |
AL |
| ZIP/Postal code |
36849 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (1) |
| GSE165969 |
Differential gene expression analysis in eosinophilic and mixed granulomatous lung inflammation |
|
