|
| Status |
Public on Mar 08, 2021 |
| Title |
Doxorubicin_921 |
| Sample type |
SRA |
| |
|
| Source name |
Normal human bronchial epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample_id: SAM24336921
cell type: NHBE
tissue: lung
treatment: Doxorubicin 500 nM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy kit.
Libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Sequencing reads were filtered and aligned using HTSeqGenie v4.2.2 against the GENCODE Basic gene model on the human genome assembly GRCh38.
Genome_build: GRCh38
Supplementary_files_format_and_content: TSV count matrices
|
| |
|
| Submission date |
Feb 02, 2021 |
| Last update date |
Mar 08, 2021 |
| Contact name |
Jason Anthony Vander Heiden |
| E-mail(s) |
vandej27@gene.com
|
| Organization name |
Genentech
|
| Department |
Biological Research AI Development
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (2) |
| GSE166033 |
RNA-seq for primary human bronchial epithelial cells, proliferating versus doxorubicin-induced senescence. |
| GSE166059 |
Molecular mapping of interstitial lung disease reveals a phenotypically distinct senescent basal epithelial cell population |
|
| Relations |
| BioSample |
SAMN17764633 |
| SRA |
SRX10008393 |
