model: Fischer 344 gender: Male age: 21 to 22 months old
Treatment protocol
N/A
Growth protocol
N/A
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using the TRIzol reagent and following the manufacturer's RNA isolation protocol (Invitrogen, #15596). One milliliter of TRIzol solution was added to each tube containing the frozen tissue block, and the tissue was homogenized by 10 passages through an 18.5 ga syringe needle. After centrifugation, the RNA was precipitated from the aqueous layer, washed, and dissolved in RNase-free water. RNA concentration and integrity were assessed by spectrophotometry and gel electrophoresis. The RNA samples were stored at 80°C.
Label
Standard Affymetrix
Label protocol
Standard Affymetrix
Hybridization protocol
Standard Affimetrix
Scan protocol
Standard Affymetrix
Description
-
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
