|
| Status |
Public on Mar 01, 2011 |
| Title |
Dnmt3L heterozygous spermatocytes 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse pachytene spermatocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
age: 8-12 weeks
tissue: Testis
genome/variation: Dnmt3L heterozygous
cell type: spermatocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
no additional information
|
| Data processing |
Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse Whole-Genome Version 1.1 BeadChips. The raw intensities were transformed by variance stabilising transformation (vst) and quantile normalised using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
|
| |
|
| Submission date |
Feb 09, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE20241 |
Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids |
|
