|
| Status |
Public on Dec 01, 2010 |
| Title |
Root Biological Replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
T34-root
|
| Organism |
Gossypium hirsutum |
| Characteristics |
gentype/variation: T34
tissue type: root
|
| Treatment protocol |
Before microarray analysis of transgenic T-34, homozygous plants were further confirmed by kanamycin-resistance screening, PCR analysis, and Southern and western blot analyses.
|
| Growth protocol |
All plants were cultured in a sterile incubator at 28˚C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were extracted from 1 g of cotton true leaves and roots from Z35 and T-34 using the Plant Total RNA Extract Kit (Autolab Biotech, Beijing, China) according to the manufacturer’s instructions.
|
| Label |
cy5
|
| Label protocol |
Fluorescent dye (Cy5 and Cy3-dCTP,ambion) labeled DNA was produced through Eberwine's linear RNA amplification method and subsequent enzymatic reaction. We took a cDNA labeling approach with Klenow enzyme after reverse transcription.
|
| |
|
| Channel 2 |
| Source name |
Z35-root
|
| Organism |
Gossypium hirsutum |
| Characteristics |
genotype/variation: Z35
tissue type: root
|
| Treatment protocol |
Before microarray analysis of transgenic T-34, homozygous plants were further confirmed by kanamycin-resistance screening, PCR analysis, and Southern and western blot analyses.
|
| Growth protocol |
All plants were cultured in a sterile incubator at 28˚C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were extracted from 1 g of cotton true leaves and roots from Z35 and T-34 using the Plant Total RNA Extract Kit (Autolab Biotech, Beijing, China) according to the manufacturer’s instructions.
|
| Label |
cy3
|
| Label protocol |
Fluorescent dye (Cy5 and Cy3-dCTP,ambion) labeled DNA was produced through Eberwine's linear RNA amplification method and subsequent enzymatic reaction. We took a cDNA labeling approach with Klenow enzyme after reverse transcription.
|
| |
|
| |
| Hybridization protocol |
standard CapitalBio Methods
|
| Scan protocol |
Luxscan 10K (CapitalBio)
|
| Description |
Root Biological replicate 2 of 3,T34(transgenic) vs Z35(control)
|
| Data processing |
After background correction and removal of bad spots, a space and intensity-dependent normalization based on a LOWESS program was employed.
|
| |
|
| Submission date |
Feb 22, 2010 |
| Last update date |
Feb 23, 2010 |
| Contact name |
miao weiguo |
| E-mail(s) |
weiguomiao1105@yahoo.com.cn
|
| Organization name |
Nanjing Agricultural Uiversity
|
| Street address |
Weigang
|
| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
|
| Platform ID |
GPL10085 |
| Series (1) |
| GSE20446 |
Hpa1Xoo-Mediated Transcriptome in Transgenic Cotton |
|
