|
| Status |
Public on Nov 02, 2021 |
| Title |
MDA-MB-468_gRNA2+dox |
| Sample type |
SRA |
| |
|
| Source name |
MDA-MB-468 breast carcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: Knockout of TCOF1
treatment: doxycyline to induce TCOF1 knockout
cell type: breast cancer cell derived mammosphere
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Isolation with TRIzol (Invitrogen)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
RNA-seq raw reads were mapped to the UCSC human reference genome (hg38) and calculation of gene counts using STAR mapping with gene quantification mode.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include raw counts for each sample
|
| |
|
| Submission date |
Mar 12, 2021 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Rebecca Y. M. Chin |
| E-mail(s) |
rebecca.chin@cityu.edu.hk
|
| Organization name |
City University of Hong Kong
|
| Department |
BMS
|
| Street address |
To Yuen Street
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE168835 |
TCOF1 DRIVES STEMNESS OF TRIPLE-NEGATIVE BREAST CANCER THROUGH KIT |
|
| Relations |
| BioSample |
SAMN18290252 |
| SRA |
SRX10335191 |
