|
Status |
Public on Nov 02, 2021 |
Title |
MDA-MB-468_gRNA2+dox |
Sample type |
SRA |
|
|
Source name |
MDA-MB-468 breast carcinoma cell line
|
Organism |
Homo sapiens |
Characteristics |
genotype: Knockout of TCOF1 treatment: doxycyline to induce TCOF1 knockout cell type: breast cancer cell derived mammosphere
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA Isolation with TRIzol (Invitrogen) RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
RNA-seq raw reads were mapped to the UCSC human reference genome (hg38) and calculation of gene counts using STAR mapping with gene quantification mode. Genome_build: hg38 Supplementary_files_format_and_content: tab-delimited text files include raw counts for each sample
|
|
|
Submission date |
Mar 12, 2021 |
Last update date |
Nov 04, 2021 |
Contact name |
Rebecca Y. M. Chin |
E-mail(s) |
rebecca.chin@cityu.edu.hk
|
Organization name |
City University of Hong Kong
|
Department |
BMS
|
Street address |
To Yuen Street
|
City |
Hong Kong |
ZIP/Postal code |
999077 |
Country |
Hong Kong |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE168835 |
TCOF1 DRIVES STEMNESS OF TRIPLE-NEGATIVE BREAST CANCER THROUGH KIT |
|
Relations |
BioSample |
SAMN18290252 |
SRA |
SRX10335191 |