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Sample GSM5213269 Query DataSets for GSM5213269
Status Public on Apr 20, 2022
Title Gata1s-FLC+miR-125b_Day10_R3
Sample type SRA
 
Source name Gata1s-FLCs
Organism Mus musculus
Characteristics treatment: Doxycycline 1ug/mL for 10 days
strain: B6J.129(Cg)-Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J
developmental stage: day E12.5-13.5 p.c.
transgene1: sgGata1
transgene2: miR-125b
Extracted molecule total RNA
Extraction protocol Quick-RNA Miniprep (Zymo Research)
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description m125_018
Data processing Raw data (raw reads) of FASTQ format were firstly processed through fastp. Clean data (clean reads) were obtained by removing reads containing adapter and poly-N sequences and reads with low quality from raw data.
hisat 2 (v2.0.5.) was used to mapp to the reference genome (--dta --phred33)
Featurecounts was used to count the read numbers mapped of each gene, including known and novel genes.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include read counts for each sample
 
Submission date Mar 26, 2021
Last update date Apr 20, 2022
Contact name Jan-Henning Klusmann
E-mail(s) jan-henning.klusmann@kgu.de
Organization name Goehte University Frankfurt
Department Pediatric Hematology and Oncology
Street address Theodor-Stern-Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platform ID GPL24247
Series (1)
GSE169739 Chromosome 21-encoded miR-125b cooperates with GATA1s in trisomy 21-associated megakaryoblastic leukemia by targeting the megakaryocytic transcription factor ARID3A II
Relations
BioSample SAMN18509979
SRA SRX10457607

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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