|
Status |
Public on Apr 20, 2022 |
Title |
Gata1s-FLC+miR-125b_Day10_R3 |
Sample type |
SRA |
|
|
Source name |
Gata1s-FLCs
|
Organism |
Mus musculus |
Characteristics |
treatment: Doxycycline 1ug/mL for 10 days strain: B6J.129(Cg)-Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J developmental stage: day E12.5-13.5 p.c. transgene1: sgGata1 transgene2: miR-125b
|
Extracted molecule |
total RNA |
Extraction protocol |
Quick-RNA Miniprep (Zymo Research) RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
m125_018
|
Data processing |
Raw data (raw reads) of FASTQ format were firstly processed through fastp. Clean data (clean reads) were obtained by removing reads containing adapter and poly-N sequences and reads with low quality from raw data. hisat 2 (v2.0.5.) was used to mapp to the reference genome (--dta --phred33) Featurecounts was used to count the read numbers mapped of each gene, including known and novel genes. Genome_build: mm10 Supplementary_files_format_and_content: tab-delimited text files include read counts for each sample
|
|
|
Submission date |
Mar 26, 2021 |
Last update date |
Apr 20, 2022 |
Contact name |
Jan-Henning Klusmann |
E-mail(s) |
jan-henning.klusmann@kgu.de
|
Organization name |
Goehte University Frankfurt
|
Department |
Pediatric Hematology and Oncology
|
Street address |
Theodor-Stern-Kai 7
|
City |
Frankfurt |
ZIP/Postal code |
60590 |
Country |
Germany |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE169739 |
Chromosome 21-encoded miR-125b cooperates with GATA1s in trisomy 21-associated megakaryoblastic leukemia by targeting the megakaryocytic transcription factor ARID3A II |
|
Relations |
BioSample |
SAMN18509979 |
SRA |
SRX10457607 |