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| Status |
Public on Mar 20, 2010 |
| Title |
dental follicle cells dexamethasone 3 |
| Sample type |
RNA |
| |
|
| Source name |
dental follicle cells after differentiation dexamethasone
|
| Organism |
Homo sapiens |
| Characteristics |
medium type: dexamethasone
cell type: dental progenitor/stem cells
|
| Treatment protocol |
Osteogenic differentiation was induced after cultivation in DMEM (PAA) supplemented with 10% foetal bovine serum (PAA), 100 µmol/L ascorbic acid 2-phosphate, 2.8 mmol/l KH2PO4, 1 × 10−7 mol/l dexamethasone sodium phosphate (Sigma-Aldrich) and HEPES (20 mmol/L). Alternatively for BMP2 or IGF2 (50 ng/mL; Sigma-Aldrich) induction of osteogenic differentiation, DFCs were cultivated in DMEM (PAA) supplemented with 1% fetal bovine serum (PAA), 100 μmol/L ascorbic acid 2-phosphate, 2.8 mmol/L KH 2 PO 4 , and HEPES (20 mmol/L).
|
| Growth protocol |
Cell were grown in in MesenchymStem Medium (PAA, Pasching, Austria) at 37°C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the kit NucleoSpin® RNA II kit (Macherey Nagel, Düren, Germany) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
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| |
|
| Hybridization protocol |
Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.0 ST array was carried out overnight with 1biotin-labeled cRNA in a hybridization oven 640
|
| Scan protocol |
GeneChip Scanner 3000 (Affymetrix)
|
| Description |
Gene expression of dental follicle cells after 7 days differentiation in dexamethasone medium
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software. For the creation of the summarized log-signal the robust multi-chip analysis (RMA) algorithm was used.
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| |
|
| Submission date |
Mar 19, 2010 |
| Last update date |
Mar 19, 2010 |
| Contact name |
Christian Morsczeck |
| E-mail(s) |
christian.morsczeck@klinik.uni-regensburg.de
|
| Organization name |
University of Regensburg
|
| Street address |
Franz-Josef Strauss Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE20963 |
Gene expression profiles of dental follicle cells after 7 days of differentiation in vitro with BMP2, IGF2 and dexamethasone |
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