|
| Status |
Public on Mar 12, 2024 |
| Title |
Control_m2 |
| Sample type |
SRA |
| |
|
| Source name |
Human liver chimeric mice
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
strain: Fah-/-/Rag2-/-/Il2rg -/- (FRG) -NOD
tissue: non-tumorous liver
age: 38 weeks
diet: CDA-HFD
treatment: Vehicle Control (PBS) i.p. 1/w for 8 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Non-tumorous liver tissue was lysed in TRI-reagent (Molecular Research Center), and RNA was purified using Direct-zol RNA MiniPrep (Zymo Research) according to the manufacturer’s instructions. RNA-Seq libraries were generated from 300 ng of total RNA
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
HNLR48
|
| Data processing |
Reads were aligned to hg19 and mm10 chromosomes concurrently using HISAT2v2.1 to separate human and mouse reads.
Human reads were further processed and normalized using DESeq2.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include DESeq2-normalized read counts for each sample
|
| |
|
| Submission date |
May 17, 2021 |
| Last update date |
Mar 12, 2024 |
| Contact name |
Frank Jühling |
| E-mail(s) |
juehling@unistra.fr
|
| Organization name |
Université de Strasbourg, Inserm, Institut de Recherche sur Les Maladies Virales et Hépatiques UMR_S1110
|
| Street address |
3 Rue Koeberlé
|
| City |
Strasbourg |
| ZIP/Postal code |
67000 |
| Country |
France |
| |
|
| Platform ID |
GPL25431 |
| Series (1) |
| GSE174543 |
Genome-wide RNAseq of liver tissue derived from humanized mice fed by CDA-HFD and treated with CLDN1 mAb or vehicle Control |
|
| Relations |
| BioSample |
SAMN19232288 |
| SRA |
SRX10910837 |
