|
| Status |
Public on May 24, 2010 |
| Title |
West Nile Virus NY99 (10^4 Copies, 200ng Human Lung RNA) 06 |
| Sample type |
RNA |
| |
|
| Source name |
WNV RNA from infected tissue culture, quantified by TaqMan Real-Time PCR
|
| Organism |
West Nile virus |
| Characteristics |
target: West Nile Virus NY99 (10^4 Copies, 200ng Human Lung RNA) 06
virus accession number: AF202541
virus taxon id: 11082
experiment type: Known Input Virus Hybridization
sample type: WNV RNA from infected tissue culture, quantified by TaqMan Real-Time PCR
|
| Treatment protocol |
viral infection
|
| Growth protocol |
infection of permissive tissue culture cell lines until cytopathic effect was observed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA extraction followed by isopropanol precipitation
|
| Label |
Cy3
|
| Label protocol |
Total RNA conversion to cDNA with degenerate oligonucleotide primers containing a known sequence tag, then amplification using primers specific to the tag that incorporate a Genisphere 3DNA-Cy3 binding sequence
|
| |
|
| Hybridization protocol |
65C overnight hybridization on a Agilent rotating incubator
|
| Scan protocol |
Agilent scanner 5um
|
| Description |
Table 1
West Nile Virus New York 1999
|
| Data processing |
Cy3 Median Signal - Background; gMedianSignal - gBGMedianSignal (Agilent data labels). No further processing, since non-parametric methods were used to analyze the data.
|
| |
|
| Submission date |
Apr 12, 2010 |
| Last update date |
May 24, 2010 |
| Contact name |
Omar Jabado |
| Organization name |
Columbia University
|
| Department |
Epidemiology
|
| Lab |
Lipkin
|
| Street address |
722 W 168 Street 17th Fl
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL10319 |
| Series (2) |
|
