|
| Status |
Public on Apr 19, 2011 |
| Title |
Rift Valley fever virus-214 |
| Sample type |
RNA |
| |
|
| Source name |
infected culture
|
| Organism |
Rift Valley fever virus |
| Characteristics |
culture: Vero or C6/36
causes hf: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Trizol extraction protocol
|
| Label |
Cy3
|
| Label protocol |
amino-allyl Cy3 dye coupled to cDNA
|
| |
|
| Hybridization protocol |
70 degree incubation for 12 hours
|
| Scan protocol |
scanned using GenePix 4000B scanner
|
| Description |
hybridization of infected culture for use in testing the performance of VIPR
|
| Data processing |
The data was produced with the intention of being analyzed by several different diagnostic algorithms, each of which employs its own normalization method. Because there is no standard normalization procedure between the different software diagnosis tools, we decided to submit raw intensities that can be used as input to any of the various available programs and independently processed by each.
|
| |
|
| Submission date |
Apr 20, 2010 |
| Last update date |
Apr 19, 2011 |
| Contact name |
Adam F Allred |
| E-mail(s) |
adam.f.allred@gmail.com
|
| Organization name |
Washington University in St. Louis, School of Medicine
|
| Street address |
660 S. Euclid
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63139 |
| Country |
USA |
| |
|
| Platform ID |
GPL10345 |
| Series (1) |
| GSE21407 |
VIPR: A probabilistic algorithm for analysis of microbial detection microarrays |
|
