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Status |
Public on Jun 10, 2010 |
Title |
Male Ethiopians Africa EthiopiaOr45a Human660W-Quad v1.0 |
Sample type |
genomic |
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Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
gender: Male population: Ethiopians region: Africa tissue: blood
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
|
Label |
C-Bio and A-DNP
|
Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
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Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
Description |
Genomic DNA extracted from blood was genotyped using Human660W-Quad v1.0 Genotyping BeadChips (Illumina).
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Data processing |
Genomic DNA extracted from blood was genotyped using Infinium Human660W-Quad v1.0 Genotyping BeadChips (Illumina). Image data was analyzed using Genomestudio v2009.2 with GT module 1.5.16 (Illumina).
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Submission date |
Apr 22, 2010 |
Last update date |
Jun 10, 2010 |
Contact name |
Mait Metspalu |
E-mail(s) |
mait@ebc.ee
|
Phone |
+37283315
|
Organization name |
Estonian Biocentre
|
Street address |
Riia 23
|
City |
Tartu |
ZIP/Postal code |
51010 |
Country |
Estonia |
|
|
Platform ID |
GPL8888 |
Series (1) |
GSE21478 |
The Genome Wide Structure of the Jewish People |
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