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Sample GSM537857

Query DataSets for GSM537857

Status

Public on Apr 29, 2010

Title

Pre-feeding 8304

Sample type

RNA

Source name

Healthy Volunteer

Organism

Homo sapiens

Characteristics

cell type: Monocytes
protocol: Pre-feeding

Extracted molecule

total RNA

Extraction protocol

Trizol extraction of total RNA was performed according to the manufacturer's instructions.

Label

biotin

Label protocol

Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

Hybridization protocol

Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Focus Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Scan protocol

GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.

Description

n/a

Data processing

The data were analyzed with GeneSpring GX11.0.1 - RMA - baseline transformation, log

Submission date

Apr 27, 2010

Last update date

Apr 28, 2010

Contact name

Stephen F Lowry

E-mail(s)

lowrysf@umdnj.edu

Phone

732-235-6096

Organization name

UMDNJ

Department

Surgery

Lab

Surgical Sciences

Street address

125 Paterson St

City

New Brunswick

State/province

ZIP/Postal code

08901

Country

USA

Platform ID

GPL201

Series (1)

GSE21534

Comparison of Enteral versus Parenteral Feeding in Healthy Human Subjects (Supported by NIH grant GM34695)

Data table header descriptions
ID_REF
VALUE	RMA, baseline transformation to median of all samples, log2

Data table
ID_REF	VALUE
AFFX-BioB-5_at	-0.1309967
AFFX-BioB-M_at	-0.12077665
AFFX-BioB-3_at	-0.25361443
AFFX-BioC-5_at	-0.026633263
AFFX-BioC-3_at	-0.0023489
AFFX-BioDn-5_at	-0.008838654
AFFX-BioDn-3_at	-0.008192062
AFFX-CreX-5_at	0.03663349
AFFX-CreX-3_at	-0.003184319
AFFX-HUMISGF3A/M97935_5_at	0.46685076
AFFX-HUMISGF3A/M97935_MA_at	0.36038828
AFFX-HUMISGF3A/M97935_MB_at	0.28134108
AFFX-HUMISGF3A/M97935_3_at	0.21988487
AFFX-HUMRGE/M10098_5_at	1.931685
AFFX-HUMRGE/M10098_M_at	2.928111
AFFX-HUMRGE/M10098_3_at	2.9270024
AFFX-HUMGAPDH/M33197_5_at	0.5173378
AFFX-HUMGAPDH/M33197_M_at	0.11372757
AFFX-HUMGAPDH/M33197_3_at	-0.08454704
AFFX-HSAC07/X00351_5_at	0.8894596

Total number of rows: 8793

Table truncated, full table size 194 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM537857_8304-pre-feeding-mono.CEL.gz	1.3 Mb	(ftp)(http)	CEL
Processed data included within Sample table