|
| Status |
Public on Jan 01, 2022 |
| Title |
3-2 |
| Sample type |
SRA |
| |
|
| Source name |
visceral adipose tissue
|
| Organism |
Canis lupus familiaris |
| Characteristics |
animal id: A4820
treatment status: Pre-ACTH
gender: F
tissue: visceral adipose tissue
|
| Treatment protocol |
Permanent administration of tetracosactide, a synthetic analog of ACTH, was performed with osmotic pumps for 25 weeks. Pumps were replaced every 4 weeks. Tetracosactide dosage started at 1.3-1.95 μg/kg/day, increasing to a final dosage of 6-10 μg/kg/day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
VAT was collected by laparotomy before and after ACTH treatment. Total RNA was isolated from VAT tissue with the RNeasy Lipid Tissue Mini Kit (Qiagen GmbH, Hilden, Germany).
The libraries were prepared following Illumina TruSeq stranded mRNA protocol. The quality of the RNA and final libraries was determined using an Agilent 4200 TapeStation System. The libraries were pooled equimolecularly and sequenced in an Illumina NovaSeq sequencer (single-end 100 bp) with a depth of around 20 Mio reads per sample.
Illumina TruSeq stranded mRNA protocol
|
| |
|
| Library strategy |
ssRNA-seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Expression values were computed using STAR and featureCounts
Genome_build: CanFam3.1
Supplementary_files_format_and_content: text files holding raw counts
|
| |
|
| Submission date |
Jun 14, 2021 |
| Last update date |
Jan 02, 2022 |
| Contact name |
Hubert Rehrauer |
| E-mail(s) |
Hubert.Rehrauer@fgcz.ethz.ch
|
| Organization name |
ETH Zurich / University of Zurich
|
| Department |
FGCZ
|
| Street address |
Winterthurerstr. 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL25760 |
| Series (1) |
| GSE178108 |
Effects of ACTH-induced long-term hypercortisolemia on the transcriptome of canine visceral adipose tissue |
|
| Relations |
| BioSample |
SAMN19693595 |
| SRA |
SRX11137215 |
