GEO help: Mouse over screen elements for information.
Status
Public on Apr 29, 2010
Title
DC.4+.MLN#3
Sample type
RNA
Source name
Mesenteric LN
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Blendzyme liberase III + DNase digestion CD3, CD19, NK1.1, GR1, Ter119 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.4+.MLN#3, mesenteric LN CD4+ dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
24.6219
10344622
158.6087
10344624
331.8777
10344633
791.1187
10344637
165.1185
10344653
17.0384
10344658
101.4602
10344674
12.6434
10344679
45.7655
10344707
164.1075
10344713
162.4773
10344723
85.1978
10344725
40.187
10344741
2950.828
10344743
25.8737
10344750
907.0024
10344772
38.2941
10344789
151.8844
10344797
449.0386
10344799
248.5276
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM538244_EA07068_91085_MoGene_DC2.MLN#3.CEL.gz
3.8 Mb
(ftp) (http) CEL
Processed data included within Sample table
