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Status
Public on Apr 29, 2010
Title
DC.8+.Sp.ST#5
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
collagenase, dispase, DNAse digestion CD3e, CD19, Gr1, NK1.1 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8+.Sp#5, spleen CD8+ dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
Jul 13, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE68399 Reanalyzed by
GSE74316 Reanalyzed by
GSE115450 Reanalyzed by
GSE117080
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.0994
10344622
210.8544
10344624
315.7321
10344633
748.3569
10344637
221.1032
10344653
16.9848
10344658
173.1879
10344674
9.3897
10344679
46.2012
10344707
170.7742
10344713
222.8096
10344723
130.3597
10344725
45.3098
10344741
3015.2115
10344743
31.9653
10344750
876.7577
10344772
41.5354
10344789
163.5443
10344797
366.3124
10344799
236.3847
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538258_EA07068_87571_MoGene_DC1.SP#5.CEL.gz
4.4 Mb
(ftp) (http) CEL
Processed data included within Sample table
