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Status
Public on Apr 29, 2010
Title
DC.8-4-11b+.MLN#5
Sample type
RNA
Source name
Mesenteric LN
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
digestion using Liberase III + DNase I CD3, CD19, GR1, NK1.1, Ter-119 using MACS beads
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8-4-11b+.MLN#5, mesenteric LN double negative dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.8787
10344622
175.0775
10344624
352.7573
10344633
864.4068
10344637
277.2188
10344653
12.513
10344658
215.561
10344674
12.0597
10344679
88.1346
10344707
234.1
10344713
185.7041
10344723
80.7941
10344725
41.7349
10344741
2166.6554
10344743
32.3782
10344750
1049.4201
10344772
31.3927
10344789
147.8124
10344797
305.9266
10344799
178.6669
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538264_EA07068_96435_MoGene_DC.8-4-11B+.MLN#5.CEL.gz
3.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
