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Status
Public on Apr 29, 2010
Title
DC.8-4-11b+.Sp#3
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Liberase Blendzymes III, DNAse I digestion CD3e, CD19, GR1, NK1.1 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8-4-11b+.Sp#3, spleen double negative dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
14.1153
10344622
274.2513
10344624
153.818
10344633
732.0027
10344637
197.6341
10344653
17.0846
10344658
123.404
10344674
13.8222
10344679
52.4484
10344707
150.6095
10344713
146.0874
10344723
191.2272
10344725
47.1007
10344741
1740.8076
10344743
35.3655
10344750
790.7271
10344772
38.7965
10344789
123.6686
10344797
1031.4042
10344799
196.2262
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM538267_EA07068_87569_MoGene_DC3.SP#3.CEL.gz
3.7 Mb
(ftp) (http) CEL
Processed data included within Sample table
