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Sample GSM538317 Query DataSets for GSM538317
Status Public on Apr 29, 2010
Title NK.Sp#9
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: CD3 CD4 CD8 CD19 Ter119 Gr-1
genetic background: C57BL/6J
phenotype markers: NK1.1+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing, FACS sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description NK.Sp#9, Splenic NK cells
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date Jun 07, 2018
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE115450

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 17.4117
10344622 181.3134
10344624 348.9306
10344633 591.1664
10344637 212.4798
10344653 22.2278
10344658 219.828
10344674 12.5677
10344679 41.0855
10344707 427.4663
10344713 141.1322
10344723 102.6566
10344725 37.1616
10344741 2157.6482
10344743 32.1982
10344750 128.83
10344772 28.2079
10344789 137.4271
10344797 506.8726
10344799 207.1146

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538317_EA07068_86163_MoGene_NK.SP#9.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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