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Status
Public on Apr 29, 2010
Title
NKT.44+NK1.1-.Th#1
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Homogenize, stain MACS enrichment, Double sort (FACS Aria)
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.44+NK1.1-.Th#1, Developing thymic iNKT cells, stage 2
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.2067
10344622
272.1256
10344624
520.4284
10344633
835.5852
10344637
256.7066
10344653
15.8144
10344658
157.3648
10344674
7.9919
10344679
38.3355
10344707
178.8941
10344713
710.2928
10344723
250.3768
10344725
33.7958
10344741
5261.9455
10344743
27.1165
10344750
291.9916
10344772
27.0498
10344789
205.8767
10344797
299.1483
10344799
245.5675
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538335_EA07068_91105_MoGene_NKT.44+NK1.1-.TH#1.CEL.gz
4.4 Mb
(ftp) (http) CEL
Processed data included within Sample table
