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Sample GSM538340 Query DataSets for GSM538340
Status Public on Apr 29, 2010
Title preB.FrD.FL#1
Sample type RNA
 
Source name Fetal Liver
Organism Mus musculus
Characteristics age: 18 days
exclusion markers: Ter119 Ly6c CD11b Gr-1 CD3
genetic background: C57BL/6J
phenotype markers: AA4.1+ IgM- CD19+ CD43- CD24+
separation method: dissection/trimming
Sex: both (pooled)
Extracted molecule total RNA
Extraction protocol ground between 2 glass slides, ACK lysis of RBCs 2x flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description preB.FrD.FL#1, Fr D (pre-B)
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.2483
10344622 301.5973
10344624 585.0187
10344633 1117.2145
10344637 310.7952
10344653 12.7285
10344658 487.9507
10344674 8.6913
10344679 34.7842
10344707 969.3422
10344713 802.1419
10344723 262.0955
10344725 38.4623
10344741 4073.7096
10344743 25.7023
10344750 245.0398
10344772 24.6195
10344789 446.5799
10344797 196.2117
10344799 455.7094

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538340_EA07068_80056_MoGene_PREB.FRD.FL#1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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