NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM538417 Query DataSets for GSM538417
Status Public on Apr 29, 2010
Title T.8Nve.Sp#3
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ 25- 62Lhi 44lo
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing, FACS sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8Nve.Sp#3, Spleen Naive CD8
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date Jul 13, 2018
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE115450
Reanalyzed by GSE117080

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.9123
10344622 220.5416
10344624 409.2292
10344633 951.5933
10344637 187.3958
10344653 15.3087
10344658 163.4197
10344674 10.6624
10344679 48.041
10344707 273.1699
10344713 480.7934
10344723 293.6705
10344725 65.0711
10344741 2347.3766
10344743 37.1772
10344750 309.7967
10344772 26.419
10344789 121.9468
10344797 423.2912
10344799 146.8294

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538417_EA07068_52755_MoGene_T.8Nve.Sp_#3.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap