|
| Status |
Public on Dec 29, 2021 |
| Title |
BMDM_LPS_3 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM
|
| Organism |
Mus musculus |
| Characteristics |
cell type: macrophage
treatment: LPS
|
| Treatment protocol |
To induce M1-like polarization, BMDM were treated with 10ng/ml LPS for 48 h in the presence or absence of HMB (1mM)
|
| Growth protocol |
bone marrow cells were isolated from C57/bl6 mice, cells were differentiated using L929 cell conditioned media as published http://abc.org.br/IMG/pdf/doc-4272.pdf
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using qiagen RNeasy Micro Kit, RNA quality was checked using an Agilent bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate sense-strand cDNA from total RNA. Following synthesis of sense-strand cDNA, the cDNA was fragmented and labeled with the kit. Fragmented and labeled cDNA was used to prepare a hybridization cocktail with the Affymetrix GeneTitan Hybridization Wash and Stain Kit for WT Arrays
|
| |
|
| Hybridization protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate was carried out using the Affymetrin GeneTitan MC Instrument.
|
| Scan protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate was carried out using the Affymetrin GeneTitan MC Instrument.
|
| Data processing |
data were normalized and batch corrected using TAC4 software
|
| |
|
| Submission date |
Jun 18, 2021 |
| Last update date |
Dec 29, 2021 |
| Contact name |
michael francis coleman |
| E-mail(s) |
mcoleman@unc.edu
|
| Phone |
9199850347
|
| Organization name |
UNC
|
| Street address |
235 dauer drive
|
| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (2) |
| GSE178475 |
Modulation of macrophage polarization by HMB [macrophage] |
| GSE178478 |
Modulation of macrophage polarization by HMB |
|
