|
| Status |
Public on Dec 29, 2021 |
| Title |
gastroc_HMB_2 |
| Sample type |
RNA |
| |
|
| Source name |
gastroc muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: gastrocnemius muscle
|
| Treatment protocol |
HMB supplementation began one week prior to tumor cell injection, with mice randomized to receive either Ctrl (AIN-93G) or Ctrl+HMB, once tumors were established following injection of 200K PANC02 cells s.c. mice were treated with 200µg anti-PD1 every 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by trizsol and RNeasy Kit (Qiagen) from homogenized tumor samples
|
| Label |
biotin
|
| Label protocol |
GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate sense-strand cDNA from total RNA. Following synthesis of sense-strand cDNA, the cDNA was fragmented and labeled with the kit. Fragmented and labeled cDNA was used to prepare a hybridization cocktail with the Affymetrix GeneTitan Hybridization Wash and Stain Kit for WT Arrays
|
| |
|
| Hybridization protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate was carried out using the Affymetrin GeneTitan MC Instrument.
|
| Scan protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate was carried out using the Affymetrin GeneTitan MC Instrument.
|
| Data processing |
data were normalized and batch corrected using TAC4 software
|
| |
|
| Submission date |
Jun 18, 2021 |
| Last update date |
Dec 29, 2021 |
| Contact name |
michael francis coleman |
| E-mail(s) |
mcoleman@unc.edu
|
| Phone |
9199850347
|
| Organization name |
UNC
|
| Street address |
235 dauer drive
|
| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (2) |
| GSE178476 |
Modulation of macrophage polarization by HMB [muscle] |
| GSE178478 |
Modulation of macrophage polarization by HMB |
|
