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Status |
Public on Apr 01, 2011 |
Title |
Temporal Cortex_Young A_M1 |
Sample type |
RNA |
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Source name |
Temporal cortex from young adult Microcebus murinus, male (1)
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Organism |
Microcebus murinus |
Characteristics |
gender: male tissue: temporal cortex age category: young
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNeasy mini kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 µg total RNA.
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Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
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Description |
Microcebus murinus were divided in 3 groups: the first group included 6 young adults (4 females and 2 males), the second included 10 healthy old animals (7 females and 3 males) and the third one was composed by 2 "AD-like" old females. Since Microcebus murinus microarrays do not exist, we decided to use Affymetrix human genome chips (HG U133 plus 2), since studies have illustrated the feasibility of detecting non-human primate brain transcripts using human genome chips.
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Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
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Submission date |
May 11, 2010 |
Last update date |
Apr 01, 2011 |
Contact name |
Gina DEVAU |
E-mail(s) |
gina.devau@umontpellier.fr
|
Phone |
33 467144830
|
Organization name |
Inserm U1198-UM-EPHE
|
Department |
Universite de Montpellier CC105
|
Lab |
Mecanismes Moleculaires dans les Demences Neurologiques (MMDN)
|
Street address |
Place Eugene Bataillon
|
City |
Montpellier |
ZIP/Postal code |
34095 cedex5 |
Country |
France |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE21779 |
Gene expression data from temporal cortex of young adult, old and AD-like Microcebus murinus |
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