|
| Status |
Public on Apr 01, 2011 |
| Title |
Temporal Cortex_Old_F3 |
| Sample type |
RNA |
| |
|
| Source name |
Temporal cortex from old Microcebus murinus, female (3)
|
| Organism |
Microcebus murinus |
| Characteristics |
gender: female
tissue: temporal cortex
age category: old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy mini kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 µg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Microcebus murinus were divided in 3 groups: the first group included 6 young adults (4 females and 2 males), the second included 10 healthy old animals (7 females and 3 males) and the third one was composed by 2 "AD-like" old females. Since Microcebus murinus microarrays do not exist, we decided to use Affymetrix human genome chips (HG U133 plus 2), since studies have illustrated the feasibility of detecting non-human primate brain transcripts using human genome chips.
|
| Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
| |
|
| Submission date |
May 11, 2010 |
| Last update date |
Apr 01, 2011 |
| Contact name |
Gina DEVAU |
| E-mail(s) |
gina.devau@umontpellier.fr
|
| Phone |
33 467144830
|
| Organization name |
Inserm U1198-UM-EPHE
|
| Department |
Universite de Montpellier CC105
|
| Lab |
Mecanismes Moleculaires dans les Demences Neurologiques (MMDN)
|
| Street address |
Place Eugene Bataillon
|
| City |
Montpellier |
| ZIP/Postal code |
34095 cedex5 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE21779 |
Gene expression data from temporal cortex of young adult, old and AD-like Microcebus murinus |
|
