|
| Status |
Public on Jun 01, 2010 |
| Title |
Astrocytes_R1 vs. Oligodendrocytes_R1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Astrocytes_R1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: Astrocytes
|
| Treatment protocol |
No treatment but different cell types were analysed.
|
| Growth protocol |
Preparation of mouse oligodendrocyte primary cultures was performed as described previously (Trajkovic et al., 2006). After 10 to 14 days, oligodendrocytes were shaken off the mixed glial cultures. Afterwards the cultures without oligodendrocytes, but containing astrocytes and microglia, were maintained in DMEM with 10% FCS. 7-10 days after shaking of oligodendrocytes another shaking of the culture released the microglia from the astrocyte layer. Microglia were harvested at this point. Primary astrocytes were harvested by trypsination after oligodendrocytes and microglia were shaken off.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Hy3
|
| Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit
|
| |
|
| Channel 2 |
| Source name |
Oligodendrocytes_R1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: Oligodendrocytes
|
| Treatment protocol |
No treatment but different cell types were analysed.
|
| Growth protocol |
Preparation of mouse oligodendrocyte primary cultures was performed as described previously (Trajkovic et al., 2006). After 10 to 14 days, oligodendrocytes were shaken off the mixed glial cultures. Afterwards the cultures without oligodendrocytes, but containing astrocytes and microglia, were maintained in DMEM with 10% FCS. 7-10 days after shaking of oligodendrocytes another shaking of the culture released the microglia from the astrocyte layer. Microglia were harvested at this point. Primary astrocytes were harvested by trypsination after oligodendrocytes and microglia were shaken off.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Hy5
|
| Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit
|
| |
|
| |
| Hybridization protocol |
The samples were hybridized on the miRCURY™ LNA Array (v.9.2) as per Exiqons protocols.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B). Images were quantified using the software 'Automic Imageprocessing for Microarrays'[Katzer, Mathias (2004) Automatisches Segmentieren von Microarraybildern. Dissertation, Faculty of Technology, Bielefeld University]
|
| Description |
Astrocytes1 vs. Oligodendrocytes_R1
|
| Data processing |
Log2-ratios between the Hy3/Hy5 have been provided. The quantified signals (background correction) were normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm using R 2.5 (limma package).
|
| |
|
| Submission date |
May 12, 2010 |
| Last update date |
Jun 01, 2010 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Pathology
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7724 |
| Series (2) |
| GSE21799 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster (miRNA Exiqon Part) |
| GSE21801 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster |
|
