|
| Status |
Public on Dec 07, 2010 |
| Title |
Ter119- fetal liver progenitors A3 |
| Sample type |
RNA |
| |
|
| Source name |
Ter119– fetal liver cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
age: Day13.5-14.5
tissue: fetal liver
cell type: Ter119- progenitor
|
| Treatment protocol |
Megakaryocytes were purified on a bovine serum albumin gradient.
|
| Growth protocol |
Day13.5-14.5 murine fetal livers were depleted of erythroid cells and cultured with murine thrombopoietin in StemSpan SFEM medium (StemCell Technologies, Vancouver, Canada) to generate megakaryocytes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent (Invitrogen, Carlsbad, CA).
|
| Label |
Hy3,Hy5
|
| Label protocol |
0.5 ug RNA was labeled using the miRCURY LNA Hy3/Hy5 labeling kit (Exiqon).
|
| |
|
| Hybridization protocol |
Hybridized to the miRCURY locked nucleic acid (LNA) array. Automated microarray hybridizations and washes were performed on a Tecan HS4800 station with 20 hr hybridization at 56ºC.
|
| Scan protocol |
Axon GenePix 400b.
|
| Description |
Geometric average of normalized 532 and 635 measurements for Sample A3.
|
| Data processing |
Normalize background-subtracted data of individual channels by loess method, truncate values less than 1 to 1.0, take geometric average of Hy3 and Hy5 measurements of each sample. The affy package R/BioConductor version 1.24.2 was used to normalize the data.
|
| |
|
| Submission date |
Jun 21, 2010 |
| Last update date |
Dec 07, 2010 |
| Contact name |
Mitchell J Weiss |
| E-mail(s) |
weissmi@email.chop.edu
|
| Phone |
215 590-0565
|
| Organization name |
The Children's Hospital of Philadelphia
|
| Lab |
316B ARC
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL7724 |
| Series (1) |
| GSE22480 |
MicroRNA expression during murine fetal liver megakaryocyte differentiation |
|
