|
| Status |
Public on Nov 30, 2010 |
| Title |
Nganassan1 |
| Sample type |
genomic |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
population: Nganassans
region: Siberia
tissue: blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
| Description |
Genomic DNA extracted from blood was genotyped using Human660W-Quad v1.0 Genotyping BeadChips (Illumina).
|
| Data processing |
Genomic DNA extracted from blood was genotyped using Infinium Human660W-Quad v1.0 Genotyping BeadChips (Illumina).
Image data was analyzed using Genomestudio v2009.2 with GT module 1.5.16 (Illumina).
|
| |
|
| Submission date |
Jun 22, 2010 |
| Last update date |
Nov 30, 2010 |
| Contact name |
Mait Metspalu |
| E-mail(s) |
mait@ebc.ee
|
| Phone |
+37283315
|
| Organization name |
Estonian Biocentre
|
| Street address |
Riia 23
|
| City |
Tartu |
| ZIP/Postal code |
51010 |
| Country |
Estonia |
| |
|
| Platform ID |
GPL8888 |
| Series (1) |
| GSE22494 |
Population genetics context of ancient human genome sequence of an extinct Palaeo-Eskimo |
|
