|
| Status |
Public on Sep 17, 2024 |
| Title |
06_KO_CT6_B |
| Sample type |
RNA |
| |
|
| Source name |
Suprachiasmatic Nuclei
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: Gpr176-/-
circadian time: CT6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression kit was used to prepare the cDNA, which was then labeled using the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
The labeled, fragmented ssDNA was hybridized to the GeneChip Mouse Gene 1.0 ST Array (Affymetrix) in a GeneChip Hybridization Oven 640 at 45°C with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
After washing and staining, the probe array was scanned using Affymetrix GeneChip Scanner 3000 7G system and the software Genechip Command Console Software (AGCC).
|
| Data processing |
The data were analyzed with the GeneSpring GX software (version 11.5.1, Agilent Technologies) in the following settings: Summarization Algorithm, RMA16; Confidence level, All; Baseline Options, Do not perform baseline transformation.
|
| |
|
| Submission date |
Sep 20, 2021 |
| Last update date |
Sep 17, 2024 |
| Contact name |
Masao Doi |
| E-mail(s) |
doimasao@pharm.kyoto-u.ac.jp
|
| Phone |
81-75753-9555
|
| Organization name |
Kyoto University, Graduate School of Pharmaceutical Sciences
|
| Department |
Department of Systems Biology
|
| Street address |
46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
6068501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE184440 |
Transcriptome profiling of circadian day and night gene expression in the suprachiasmatic nucleus of wild-type and Gpr176-deficient mice |
|
