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Status |
Public on May 14, 2012 |
Title |
HCC-1428/LTED, EtOH, rep 3 |
Sample type |
RNA |
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Source name |
HCC-1428/LTED cells, EtOH
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCC-1428/LTED cell type: breast cancer treatment: ethanol
|
Treatment protocol |
Cells were seeded in 100-mm dishes in triplicate. The following day, cells were washed with PBS, and treated with IMEM + 10% DCC-FBS (no phenol red) containing 0.1% EtOH or 1 uM fulvestrant. Forty-eight hrs later, RNA was harvested.
|
Growth protocol |
MCF-7/LTED and HCC-1428/LTED cells were maintained in IMEM + 10% DCC-FBS (no phenol red).
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion.
|
Label |
biotin
|
Label protocol |
GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix).
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|
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Hybridization protocol |
15 ug biotinylated cRNA was fragmented and hybridizied to an Affymetrix GeneChip Human Genome U133 Plus 2.0 array.
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Scan protocol |
Hybridized cRNA was detected using streptavidin coupled to phycoerythrin. GeneChips were scanned using the GeneChip Scanner 3000 7G Plus 2 and GeneChip Operating System (Affymetrix).
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Description |
Gene expression data from HCC-1428/LTED cells treated with 10% DCC-FBS + 0.1% EtOH x 48 hrs.
|
Data processing |
Default values were used to grid images (.DAT) and generate .CEL and .CHP files. Data were RMA-normalized using Expression Console (Affymetrix).
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Submission date |
Jun 23, 2010 |
Last update date |
May 14, 2012 |
Contact name |
Todd W Miller |
E-mail(s) |
todd.miller@vanderbilt.edu
|
Organization name |
Vanderbilt Univ
|
Department |
Medicine
|
Street address |
2220 Pierce Ave, 777 PRB
|
City |
Nashville |
State/province |
TN |
ZIP/Postal code |
37174 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE22533 |
Breast cancer cells resistant to hormone deprivation maintain an estrogen receptor alpha-dependent, E2F-directed transcriptional program |
GSE37955 |
ERa-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer |
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