|
| Status |
Public on Nov 16, 2011 |
| Title |
Kavya with GMB4M, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
stem, GMB4M
|
| Organism |
Oryza sativa |
| Characteristics |
variety: Kavya
tissue: stem
infestation: gall midge biotype 4M (GMB4M)
|
| Treatment protocol |
15-day-old kavya seedlngs were exposed to gall midge biotype GMB1 or GMB4M for 24, 48, 96, and 120 hr. Uninfested seedlings were used as control.
|
| Growth protocol |
Samples were grown under standard glasshouse conditions for 15 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from the Kavya seedlings was extracted after removing the maggots at different time intervals. The Trizol (Invitogen) method for RNA isolation was used for the extractions. RNA was further purified using the Qiagen mini RNA isolation kit.
|
| Label |
biotin
|
| Label protocol |
The kit supplied with Affymetrix chips was used to label the RNA with biotin.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned using the GeneChip Scanner 3000.
|
| Description |
Pooled samples 24, 48, 96, and 120 hr after GMB4M infestation.
|
| Data processing |
MAS5.0 using GCOS 1.x.
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Nov 16, 2011 |
| Contact name |
Nidhi Rawat |
| E-mail(s) |
nidhi_rawat608@yahoo.co.in
|
| Phone |
+91-9346856312
|
| Organization name |
Directorate of rice research
|
| Department |
entomology
|
| Lab |
host plant resistant lab
|
| Street address |
Rajendranager
|
| City |
Hyderabad |
| State/province |
Andhra Pradesh |
| ZIP/Postal code |
500030 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE22538 |
Differential expression for rice-gall midge interaction |
|
