|
| Status |
Public on Jan 01, 2022 |
| Title |
Cur rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Gingiva
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: Gingiva
age: Six-week-old
genotype: wild type
treatment: Experimental periodontitis treated by curcumin hydrogel
|
| Treatment protocol |
EP was established by ligature with 4–0 silk thread around the bilateral maxillary first molars. Three days after ligature, P. gingivalis ATCC 33277 (107 CFU/mL) was smeared onto silk twice. The method of administration was to inject 200 μL each hydrogel into the periodontal pocket of bilateral maxillary first molar with a blunt syringe, once a week for 4 weeks. Hydrogel was used as a control for sham group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol® reagent (Thermo Fisher Scientific, Inc.).
The TruSeq Stranded total RNA with RiboZero Plus kit (IIlumina, San Diego, CA) was used to obtain a sequencing library from 1μg of total RNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Eukaryotic mRNA sequencing was performed on Illumina Novaseq 6000 by Shanghai Majorbio Bio-pharm Technology Co., Ltd. Raw gene counts with a minimum of two counts per million in at least one sample were used for downstream analyses. Differentially expressed genes were determined by the DESeq2 Bioconductor/R package.
Genome_build: Rnor_6.0
Supplementary_files_format_and_content: tab-delimited text files include gene count for each Sample ...
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Oct 01, 2021 |
| Last update date |
Jan 01, 2022 |
| Contact name |
CHENGCHENG LIU |
| E-mail(s) |
liuchengchengsc@gmail.com
|
| Organization name |
Sichuan University
|
| Street address |
No. 14, Section 3, Renminnan Road
|
| City |
CHENGDU |
| State/province |
SICHUAN |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE185147 |
The effect of curcumin/ZNPs hydrogel on the transcription of gingiva in rats with periodontitis |
|
| Relations |
| BioSample |
SAMN21971189 |
| SRA |
SRX12433825 |
