|
Status |
Public on Jan 01, 2022 |
Title |
Cur rep2 |
Sample type |
SRA |
|
|
Source name |
Gingiva
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague-Dawley tissue: Gingiva age: Six-week-old genotype: wild type treatment: Experimental periodontitis treated by curcumin hydrogel
|
Treatment protocol |
EP was established by ligature with 4–0 silk thread around the bilateral maxillary first molars. Three days after ligature, P. gingivalis ATCC 33277 (107 CFU/mL) was smeared onto silk twice. The method of administration was to inject 200 μL each hydrogel into the periodontal pocket of bilateral maxillary first molar with a blunt syringe, once a week for 4 weeks. Hydrogel was used as a control for sham group.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with TRIzol® reagent (Thermo Fisher Scientific, Inc.). The TruSeq Stranded total RNA with RiboZero Plus kit (IIlumina, San Diego, CA) was used to obtain a sequencing library from 1μg of total RNA.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Eukaryotic mRNA sequencing was performed on Illumina Novaseq 6000 by Shanghai Majorbio Bio-pharm Technology Co., Ltd. Raw gene counts with a minimum of two counts per million in at least one sample were used for downstream analyses. Differentially expressed genes were determined by the DESeq2 Bioconductor/R package. Genome_build: Rnor_6.0 Supplementary_files_format_and_content: tab-delimited text files include gene count for each Sample ... Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
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|
|
Submission date |
Oct 01, 2021 |
Last update date |
Jan 01, 2022 |
Contact name |
CHENGCHENG LIU |
E-mail(s) |
liuchengchengsc@gmail.com
|
Organization name |
Sichuan University
|
Street address |
No. 14, Section 3, Renminnan Road
|
City |
CHENGDU |
State/province |
SICHUAN |
ZIP/Postal code |
610041 |
Country |
China |
|
|
Platform ID |
GPL25947 |
Series (1) |
GSE185147 |
The effect of curcumin/ZNPs hydrogel on the transcription of gingiva in rats with periodontitis |
|
Relations |
BioSample |
SAMN21971189 |
SRA |
SRX12433825 |