|
| Status |
Public on Oct 07, 2021 |
| Title |
LI_MBELN2 |
| Sample type |
RNA |
| |
|
| Source name |
B6 Colon tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: colon
treatment: mice orally gavaged with MBELN for 7 days
|
| Treatment protocol |
10 x 10E+09 particle/100 µl MBELN (Mulberry, an edible plant, bark derived exosomes-like nanoparticles) per dose were orally given to each mice every day for 7 days. After 7 days mice were sacrificed and organs (colon) tissues were harvested for RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 90 ng total RNA (IonAmpliseq Trancriptome Mouse gene expression).
|
| |
|
| Hybridization protocol |
Following fragmentation, 100 ng of cRNA were hybridized for 16 hr at 45C on GeneChip™ Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip™ Scanner 3000 7G
|
| Data processing |
Transcriptome analysis console 4.0 was used to analyze the data.
gc-correction,scale-intensities,rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish
|
| |
|
| Submission date |
Oct 05, 2021 |
| Last update date |
Oct 07, 2021 |
| Contact name |
Mukesh Sriwastva |
| E-mail(s) |
sriwastva.mukesh6@gmail.com
|
| Phone |
5028528624
|
| Organization name |
University of Louisville
|
| Street address |
505 S Hancock St
|
| City |
LOUISVILLE |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (2) |
| GSE185351 |
Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Colon] |
| GSE185352 |
Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days |
|
