|
| Status |
Public on Jul 25, 2022 |
| Title |
yox1_9A_yhp1_13A_A_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
yox1_9A_yhp1_13A_A_rep2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: YBL261
genotype: MATa ura3delta0 leu2delta0 his3delta0 met15delta0 yox1-9A-3V5-KanMx yhp1-13A-13MYC-LEU2
|
| Treatment protocol |
none
|
| Growth protocol |
RNA was purified from asynchronous cells growing in rich medium (YM-1 with 2% dextrose).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using an acid-phenol extraction protocol.
polyA depletion and library construction were performed by BGI Americas.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
MATa ura3∆0 leu2∆0 his3∆0 met15∆0 yox1-9A-3V5-KanMx yhp1-13A-13MYC-LEU2
tpm.constitutive.phos.0718.csv
|
| Data processing |
aligned to Saccharomyces_cerevisiae R64-1-1 genome, with R64-1-1.90 annotation, by STAR(v2.5.3a) aligner.
Gene expression were quantified with featureCounts v1.5.2, measuring exon reads for each gene_id.
TPM was calculated
Genome_build: R64-1-1
Supplementary_files_format_and_content: TPM was calculated
|
| |
|
| Submission date |
Nov 04, 2021 |
| Last update date |
Jul 25, 2022 |
| Contact name |
Jennifer Benanti |
| E-mail(s) |
Jennifer.Benanti@umassmed.edu
|
| Phone |
508-856-1773
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Street address |
364 Plantation St., LRB 525
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL25927 |
| Series (2) |
| GSE188177 |
Identification of genes regulated by Yox1 and Yhp1 in S. cerevisiae |
| GSE188180 |
Identification of Yox1 and Yhp1 target genes in S. cerevisiae |
|
| Relations |
| BioSample |
SAMN22888116 |
| SRA |
SRX12971227 |
