|
| Status |
Public on Jul 25, 2022 |
| Title |
Z3EVp_YHP1_60_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Z3EVp_YHP1_60_rep1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: YMC230
genotype: MATa ura3delta0 his3delta1 lypdelta0 can1delta::STE2pr-SpHIS5 URA3::Z4pr-YHP1-3V5-KanMx HAP1+::NatMX::pACT1-Z3EV-ENO2term
|
| Treatment protocol |
1μM estradiol was added for 20 or 60 minutes, as indicated.
|
| Growth protocol |
RNA was purified from asynchronous cells growing in rich medium (YM-1 with 2% dextrose) without estradiol addition (0 minutes), and 20 or 60 minutes following the addition of estradiol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using an acid-phenol extraction protocol.
polyA depletion and library construction were performed by BGI Americas.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
MATa ura3∆0 his3∆1 lyp∆0 can1∆::STE2pr-SpHIS5 URA3::Z4pr-YHP1-3V5-KanMx HAP1+::NatMX::pACT1-Z3EV-ENO2term
TPM.inducible.yhp1.xlsx
|
| Data processing |
aligned to Saccharomyces_cerevisiae R64-1-1 genome, with R64-1-1.90 annotation, by STAR(v2.5.3a) aligner.
Gene expression were quantified with featureCounts v1.5.2, measuring exon reads for each gene_id.
TPM was calculated
Genome_build: R64-1-1
Supplementary_files_format_and_content: TPM was calculated
|
| |
|
| Submission date |
Nov 04, 2021 |
| Last update date |
Jul 25, 2022 |
| Contact name |
Jennifer Benanti |
| E-mail(s) |
Jennifer.Benanti@umassmed.edu
|
| Phone |
508-856-1773
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Street address |
364 Plantation St., LRB 525
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL25927 |
| Series (2) |
| GSE188179 |
Identification of genes regulated by acute overexpression of Yox1 and Yhp1 in S. cerevisiae |
| GSE188180 |
Identification of Yox1 and Yhp1 target genes in S. cerevisiae |
|
| Relations |
| BioSample |
SAMN22888909 |
| SRA |
SRX12971282 |
