|
| Status |
Public on Jul 31, 2010 |
| Title |
Liver_hyperglycima_no.4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, fasting
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
gender: female
disease status: type 2 diabetes
|
| Treatment protocol |
Patients with type 2 diabetes were treated with diet therapy alone or insulin; no patients were receiving any oral hypoglycaemic agent. Participants receiving statins, ACE inhibitors or angiotensin II receptor blockers were excluded.
|
| Growth protocol |
Hepatic tissues were obtained with percutaneous needle liver biopsy, and immediately frozen in liquid nitrogen and stored at −80°C until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with a kit (Micro RNA Isolation Kit; Stratagene, La Jolla, CA, USA), purified with RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
A total of 100 ng of total RNA was labelled according to the small sample labelling protocol recommended by the manufacturer.
|
| |
|
| Hybridization protocol |
Ten micrograms of labelled and fragmented cRNA was hybridised onto a Human Genome U133 Plus 2.0 Array (Affymetrix) for 16 h at 45°C.
|
| Scan protocol |
Chips were scanned by using GeneChip Scanner3000, Affymetrix.
|
| Description |
Gene expression data from human liver.
|
| Data processing |
A scanned image was quantified using GCOS version 1.1.1 (GeneChip Operating Software; Affymetrix) with the default parameters for the statistical algorithm and all probe sets scaled to a target intensity of 100.
|
| |
|
| Submission date |
Jul 30, 2010 |
| Last update date |
Jul 30, 2010 |
| Contact name |
Hirofumi Misu |
| E-mail(s) |
hmisu@m-kanazawa.jp
|
| Phone |
+81-76-265-2234
|
| Organization name |
Kanazawa University
|
| Street address |
13-1 Takaramachi
|
| City |
Kanazawa |
| ZIP/Postal code |
920-8641 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23343 |
Expression data from human liver with or without type 2 diabetes |
|
