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Status |
Public on Dec 17, 2021 |
Title |
Laos27 |
Sample type |
genomic |
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Source name |
blood
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Organism |
Homo sapiens |
Characteristics |
population: Lao region: Southeast Asia tissue: blood
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
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Label |
Cy3, Cy5
|
Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
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Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48°C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
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Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol, dried for 1 hour and scanned on a BeadArray Reader (Illumina).
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Description |
Genomic DNA extracted from blood was genotyped using HumanOmniExpress 24 v1.1 BeadChip (Illumina).
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Data processing |
Image data was analyzed using Genomestudio v2.0 with GT module 2.0.4 (Illumina). Genotype: AA,AB,BB,or NC (no call)
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Submission date |
Dec 15, 2021 |
Last update date |
Dec 17, 2021 |
Contact name |
Tuuli Reisberg |
Organization name |
University of Tartu
|
Street address |
Riia 23
|
City |
Tartu |
ZIP/Postal code |
51010 |
Country |
Estonia |
|
|
Platform ID |
GPL22678 |
Series (1) |
GSE190958 |
On the genetic constitution of the Marquesas |
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